Linear poly(methyl glycerol) and linear polyglycerol as potent protein and cell resistant alternatives to poly(ethylene glycol)

authored by: Marie Weinhart, Ingo Grunwald, Monika Wyszogrodzka, Linda Gaetjen, Andreas Hartwig, Rainer Haag
Abstract: The nonspecific interaction of proteins with surfaces in contact with biofluids leads to adverse problems and is prevented by a biocompat-ible surface coating. The current benchmark material among such coatings is poly(ethylene glycol) (PEG). Herein, we report on the synthesis of linear polyglycerol derivatives as promising alternatives to PEG. Therefore, gold surfaces as a model system are functionalized with a self-assembled monolayer (SAM) by a two-step anhydride coupling and a direct thiol immobilization of linear poly(methyl glycerol) and polyglycerol. Surface plasmon resonance (SPR) spectroscopy reveals both types of functionalized surfaces to be as resistant as PEG towards the adsorption of the test proteins fibrinogen, pepsin, albumin, and lysozyme. Moreover, linear polyglycerols adsorb even less proteins from human plasma than a PEG-modified surface. Additional cell adhesion experiments on linearpoly(methyl glycerol) and polyglycerol-modified surfaces show comparable cell resistance as for a PEG-modified surface. Also, in the case of long-term stability, high cell resistance is observed for all samples in medium. Additional in vitro cell-toxicity tests add to the argument that linear poly(methyl glycer-ol) and polyglycerol are strong candidates for promising alternatives to PEG, which can easily be modified for biocompatible functionalization of other surfaces.
External Organisation(s): Freie Universität Berlin (FU Berlin)
Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM)
TU Dortmund University
Type: Article
Journal: Chemistry - An Asian Journal
Volume: 5
Pages: 1992-2000
No. of pages: 9
ISSN: 1861-4728
Publication date: 02.07.2010
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biochemistry, Organic Chemistry
Electronic version(s): https://doi.org/10.1002/asia.201000127 (Access: Closed)

BibTeX

@article{384095410fae4c879f60ceb4dce913dd,
title = "Linear poly(methyl glycerol) and linear polyglycerol as potent protein and cell resistant alternatives to poly(ethylene glycol)",
abstract = "The nonspecific interaction of proteins with surfaces in contact with biofluids leads to adverse problems and is prevented by a biocompat-ible surface coating. The current benchmark material among such coatings is poly(ethylene glycol) (PEG). Herein, we report on the synthesis of linear polyglycerol derivatives as promising alternatives to PEG. Therefore, gold surfaces as a model system are functionalized with a self-assembled monolayer (SAM) by a two-step anhydride coupling and a direct thiol immobilization of linear poly(methyl glycerol) and polyglycerol. Surface plasmon resonance (SPR) spectroscopy reveals both types of functionalized surfaces to be as resistant as PEG towards the adsorption of the test proteins fibrinogen, pepsin, albumin, and lysozyme. Moreover, linear polyglycerols adsorb even less proteins from human plasma than a PEG-modified surface. Additional cell adhesion experiments on linearpoly(methyl glycerol) and polyglycerol-modified surfaces show comparable cell resistance as for a PEG-modified surface. Also, in the case of long-term stability, high cell resistance is observed for all samples in medium. Additional in vitro cell-toxicity tests add to the argument that linear poly(methyl glycer-ol) and polyglycerol are strong candidates for promising alternatives to PEG, which can easily be modified for biocompatible functionalization of other surfaces.",
keywords = "Adsorption, Cell adhesion, Monolayers, Proteins, Self-assembly",
author = "Marie Weinhart and Ingo Grunwald and Monika Wyszogrodzka and Linda Gaetjen and Andreas Hartwig and Rainer Haag",
year = "2010",
month = jul,
day = "2",
doi = "10.1002/asia.201000127",
language = "English",
volume = "5",
pages = "1992--2000",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "John Wiley and Sons Ltd",
number = "9",
}