Establishment and optimization of an efficient in vitro regeneration system of oncidium, wilsonara, odontocidium and vuylstekeara

authored by: B. Raffeiner, M. Serek, T. Winkelmann
Abstract: Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance. The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis. Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar 'SP 12' and Sharry Baby var. aureum 'OK', as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria 'Plush', we established a reliable protocol for regeneration of somatic embryos. The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks. The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
Organisation(s): Institute of Horticultural Production Systems
External Organisation(s): Nursery Valtl Raffeiner
Type: Conference contribution
Pages: 445-452
No. of pages: 8
Publication date: 29.10.2010
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Horticulture
Electronic version(s): https://doi.org/10.17660/ActaHortic.2010.878.57 (Access: Closed)

BibTeX

@inproceedings{1f72822a2cf94112b9f2882f7d99dbf0,
title = "Establishment and optimization of an efficient in vitro regeneration system of oncidium, wilsonara, odontocidium and vuylstekeara",
abstract = "Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance. The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis. Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar 'SP 12' and Sharry Baby var. aureum 'OK', as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria 'Plush', we established a reliable protocol for regeneration of somatic embryos. The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks. The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.",
keywords = "Embryogenesis, Liquid culture, Micropropagation, Orchids, Somatic, Thidiazuron",
author = "B. Raffeiner and M. Serek and T. Winkelmann",
year = "2010",
month = oct,
day = "29",
doi = "10.17660/ActaHortic.2010.878.57",
language = "English",
isbn = "9789066055735",
series = "Acta Horticulturae",
publisher = "International Society for Horticultural Science",
pages = "445--452",
booktitle = "I International Orchid Symposium",
address = "Belgium",
}