Purification and sequencing of cytochrome b from potato reveals methionine cleavage of a mitochondriallly encoded protein

verfasst von: Hans-Peter Braun, Udo Schmitz
Abstract: Several mitochondrial genes from a large number of different fungi, mammals and plants have been sequenced but little is known about the corresponding translation products. We have affinity purified cytochrome c reductase from potato mitochondria and isolated the mitochondrially encoded cytochrome b protein. Amino-terminal sequencing reveals that the polypeptide does not start with a methionine. Comparison of the amino acid sequence with the recently published sequence of the gene encoding the cytochrome b apoprotein suggests that the N-fonnylmetnionine is removed. This result provides the first evidence for the presence of a deformylase and a methionine aminopeptidase in mitochondria.
Externe Organisation(en): Max-Planck-Institut für molekulare Pflanzenphysiologie
Typ: Artikel
Journal: FEBS letters
Band: 316
Seiten: 128-132
Anzahl der Seiten: 5
ISSN: 0014-5793
Publikationsdatum: 25.01.1993
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Biophysik, Strukturelle Biologie, Biochemie, Molekularbiologie, Genetik, Zellbiologie
Elektronische Version(en): https://doi.org/10.1016/0014-5793(93)81200-J (Zugang: Unbekannt)

BibTeX

@article{ddc93c7e35c945088b6dd2b1893f853b,
title = "Purification and sequencing of cytochrome b from potato reveals methionine cleavage of a mitochondriallly encoded protein",
abstract = "Several mitochondrial genes from a large number of different fungi, mammals and plants have been sequenced but little is known about the corresponding translation products. We have affinity purified cytochrome c reductase from potato mitochondria and isolated the mitochondrially encoded cytochrome b protein. Amino-terminal sequencing reveals that the polypeptide does not start with a methionine. Comparison of the amino acid sequence with the recently published sequence of the gene encoding the cytochrome b apoprotein suggests that the N-fonnylmetnionine is removed. This result provides the first evidence for the presence of a deformylase and a methionine aminopeptidase in mitochondria.",
keywords = "Cytochrome b, Cytochrome c reductase, Methionine aminopeptidase, Mitochondria, Solanum tuberosum",
author = "Hans-Peter Braun and Udo Schmitz",
note = "Funding information: AcknowledgemenWtse: are greatlyi ndeptedt o Dr. V. Kruft, Berlin, for sequencingth e cytochromeb protein.W e wish to thankP rof. G. Schatz,B asel,w ho madea ntibodiesa gainstc ytochromebs from yeast available to us. This work was supported by the Deutsche Forschungsgemeinscha(Sftc hm6 98/2-2).",
year = "1993",
month = jan,
day = "25",
doi = "10.1016/0014-5793(93)81200-J",
language = "English",
volume = "316",
pages = "128--132",
journal = "FEBS letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "2",
}