Automated imunoanalysis systems for monitoring mammalian cell cultivation processes

verfasst von: Birgitt Schulze, Cornelia Middendorf, Martin Reinecke, Thomas Scheper, Wolfgang Noé, Michael Howaldt
Abstract: Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
Externe Organisation(en): Westfälische Wilhelms-Universität Münster (WWU)
Typ: Artikel
Journal: CYTOTECHNOLOGY
Band: 15
Seiten: 259-269
Anzahl der Seiten: 11
ISSN: 0920-9069
Publikationsdatum: 02.1994
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Biotechnologie, Bioengineering, Biomedizintechnik, Klinische Biochemie, Zellbiologie
Elektronische Version(en): https://doi.org/10.1007/BF00762401 (Zugang: Unbekannt)

BibTeX

@article{7959d14f34784101a5e01b01e0d7156e,
title = "Automated imunoanalysis systems for monitoring mammalian cell cultivation processes",
abstract = "Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.",
keywords = "Automated immunoanalysis, flow-injection analysis, heterogeneous assay, process analysis, turbidimetric assay",
author = "Birgitt Schulze and Cornelia Middendorf and Martin Reinecke and Thomas Scheper and Wolfgang No{\'e} and Michael Howaldt",
year = "1994",
month = feb,
doi = "10.1007/BF00762401",
language = "English",
volume = "15",
pages = "259--269",
journal = "CYTOTECHNOLOGY",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "1-3",
}