Rapid capillary gel electrophoresis of proteins
- verfasst von
- R. Lausch, T. Scheper, O. W. Reif, J. Schlösser, J. Fleischer, R. Freitag
- Abstract
The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses (Mr) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with Mr in the range 29 000-97 400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm. A linear relationship between migration time and log Mr was found and rapid determination of the molecular mass of light and heavy chains of human immunoglobulin G is reported. The results are compared with applications using longer separation distances, showing that rapid and efficient analysis and adequate resolution can be obtained by using short separation distances.
- Externe Organisation(en)
-
Westfälische Wilhelms-Universität Münster (WWU)
- Typ
- Artikel
- Journal
- Journal of Chromatography A
- Band
- 654
- Seiten
- 190-195
- Anzahl der Seiten
- 6
- ISSN
- 0021-9673
- Publikationsdatum
- 12.11.1993
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Analytische Chemie, Biochemie, Organische Chemie
- Elektronische Version(en)
-
https://doi.org/10.1016/0021-9673(93)83080-C (Zugang:
Unbekannt)
