Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L

verfasst von: Carsten Zeilinger
Abstract: Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.
Organisationseinheit(en): Abteilung Zellphysiologie und Biophysik
Typ: Artikel
Journal: FEBS letters
Band: 348
Seiten: 278-82
Anzahl der Seiten: 5
ISSN: 0014-5793
Publikationsdatum: 18.07.1994
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
Elektronische Version(en): https://doi.org/10.1016/0014-5793(94)00629-6 (Zugang: Unbekannt)

BibTeX

@article{666b1f92b7834f94aa443cfc6e79128e,
title = "Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L",
abstract = "Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.",
keywords = "Biological Transport/drug effects, Cell Membrane/chemistry, Cell Membrane Permeability, Electric Conductivity, Fabaceae/chemistry, Kinetics, Lipid Bilayers/metabolism, Membrane Potentials, Plants, Medicinal, Potassium/pharmacology, Potassium Channels/drug effects, Rubidium Radioisotopes, Tetraethylammonium, Tetraethylammonium Compounds/pharmacology",
author = "Carsten Zeilinger",
year = "1994",
month = jul,
day = "18",
doi = "10.1016/0014-5793(94)00629-6",
language = "English",
volume = "348",
pages = "278--82",
journal = "FEBS letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "3",
}