Calorimetric biosensor for the detection and determination of enantiomeric excesses in aqueous and organic phases

verfasst von

H. G. Hundeck, M. Weiß, T. Scheper, F. Schubert

Abstract

A new principle of a calorimetric biosensor for the determination of enantiomeric excesses is described. Two enzymes, immobilized on polymer resins, are used in the system. One hydrolyses only one enantiomer while the other converts both. It is thus possible to determine the concentration of the analyte as well as the ratio of L to D enantiomer. The sensor was tested in aqueous solutions containing up to 50% dimethyl formamide (DMF), and was successfully applied to the analysis of enantiomeric excesses. This result will open novel applications of biosensors for monitoring enzymatic reactions, even when organic solvents are used.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Physikalisch-Technische Bundesanstalt (PTB)

Typ

Artikel

Journal

Biosensors and Bioelectronics

Band

8

Seiten

205-208

Anzahl der Seiten

4

ISSN

0956-5663

Publikationsdatum

1993

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Biophysik, Biomedizintechnik, Elektrochemie

Elektronische Version(en)

https://doi.org/10.1016/0956-5663(93)85034-L (Zugang: Unbekannt)