A bio-FET sensor for lactose based on co-immobilized β-galactosidase/glucose dehydrogenase

verfasst von

Fortunato Sevilla, Thomas Kullick, Thomas Scheper

Abstract

A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD⁺ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.

Externe Organisation(en)

University of Santo Tomas
Westfälische Wilhelms-Universität Münster (WWU)

Typ

Artikel

Journal

Biosensors and Bioelectronics

Band

9

Seiten

275-281

Anzahl der Seiten

7

ISSN

0956-5663

Publikationsdatum

1994

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Biophysik, Biomedizintechnik, Elektrochemie

Elektronische Version(en)

https://doi.org/10.1016/0956-5663(94)80024-3 (Zugang: Unbekannt)